Presenting MauriceFlex, a novel protein fractionation system that enables users to collect fractions for the analysis of charge variants. Users of MauriceFlex receive:
- The ability to work with fractions more freely: analyze with intact mass, peptide mapping, reduced and subunit masses, and more.
- Release from arduous high-performance liquid chromatography (HPLC) and ion-exchange chromatography (IEX) procedures: readily collect high-purity fractions without the need for further, time-consuming chromatography-based fractionation development.
- The ability to accomplish anything on one’s own: imaged-capillary isoelectric focusing (icIEF), CE-SDS, and icIEF protein fractionation all in one instrument.
- The ability to use time more freely: charge variations can be separated, fractionated, and identified in less than a day.
MauriceFlex: icIEF, CE-SDS, and icIEF Fractionation
Video Credit: Bio-Techne Inc.
The MauriceFlex integrates icIEF, CE-SDS analysis, and protein charge isoform fractionation into one unit, enabling users to go beyond routine charge and size and perform charge variant analysis of their molecule at any point without requiring the use of separate techniques like IEX and HPLC.
Using IEX or HPLC for protein fractionation?
Compared to IEX, HPLC, and other existing technologies, protein fractionation with MauriceFlex yields considerable time and capital savings. The principal benefits are:
Image Credit: Bio-Techne Inc.
- Same-day fraction collection
- High purity fractions
- Compatibility with methods containing methylcellulose and urea
- One fractionation run providing sufficient material for intact and reduced mass analysis
- Ability to pool fractions from multiple runs for peptide mapping
- The same system offering CE-SDS and cIEF assays
icIEF protein fractionation workflow with MauriceFlex
Image Credit: Bio-Techne Inc.
How protein fractionation works on MauriceFlex
- Exposure to an electrical field produces a pH gradient across the capillary within the MauriceFlex cartridge.
- Proteins separate by moving to different pH values on the gradient, based on their unique isoelectric point (pI).
- When ammonium acetate is added, the pH gradient is altered, which mobilizes the divided proteins and causes elution (fractionation).
Image Credit: Bio-Techne Inc.
A. icIEF profiles of charge isoforms. B. Mobilization of charge isoform peaks for fractionation. Image Credit: Bio-Techne Inc.
Which Maurice is right for the user?
Source: Bio-Techne Inc
| System |
Maurice |
Maurice C. |
Maurice S. |
Maurice Flex |
| icIEF Charge Application |
Yes |
Yes |
No |
Yes |
| CE-SDS Size Application |
Yes |
No |
Yes |
Yes |
| icIEF Fractionation |
No |
No |
No |
Yes |
| Absorbance Detection |
Yes |
Yes |
Yes |
Yes |
| Fluorescence Detection |
Yes |
Yes |
No |
Yes |
| Onboard Mixing for Sample Prep |
Yes |
Yes |
No |
No |
Specifications
Source: Bio-Techne Inc
| Description |
cIEF |
CE-SDS PLUS |
Turbo CE-SDS |
cIEF Fractionation |
| Minimum Sample Volume |
50 µL |
50 µL |
100 µL |
100 µL |
| Sample Delivery |
Vacuum |
Electrokinetic |
Electrokinetic |
Vacuum |
| Typical Separation Time |
6-10 min (molecule-dependent) |
Reduced IgG: 25 minutes; Non-reduced IgG: 35 minutes |
Reduced IgG: 5.5 minutes; Non-reduced IgG: 8 minutes |
40 – 50 min (molecule-dependent) |
| Detection Capability |
UV Absorbance at 280 nm
Fluorescence: Ex 280 nm, Em 320–450 nm |
UV Absorbance at 220 nm |
UV Absorbance at 220 nm |
Fluorescence: Ex 280 nm, Em 320-450 nm |
| Typical Voltage |
Pre-focusing: 1500 V
Focusing: 3000 V |
Separation: 5750 V |
Separation: 4,200 V |
Pre-focusing: 500 V and 1000 V
Focusing: 1500 V |
| Sample Injections per Cartridge |
100 guaranteed, 200 maximum (max 25 batches) |
100 guaranteed, 500 maximum (max 25 batches) |
100 guaranteed (max 25 batches) |
Maximum 15 injections |
| Maximum Sample Injections per Batch |
100 |
48 |
96 |
1 (fractionation)
4 (cIEF)
|
| pI/Size Range |
2.85-10.45 |
10-270 kDa |
10-270 kDa |
3-10 |
| pI/Sizing CV |
1 % |
≤2 % |
<2 % |
1 % |
| CV for Peaks >10 % Composition |
≤5 % (Intra-batch), ≤6 % (Inter-batch) |
N/A |
N/A |
≤10 % (Inter-batch) |
| Relative Migration Time CV |
N/A |
<1 % for reduced IgG |
<5 % |
N/A |
| pI/Sizing Resolution |
0.05 pl units (for wide range 3-10 ampholyte) |
≥ 1.5 for NGHC/HC IgG Standard) |
≥ 1.0 for NGHC/HC IgG Standard) |
N/A |
| Dynamic Range |
2 logs |
2 logs |
2 logs |
N/A |
| Linearity |
>0.995 |
>0.995 |
>0.995 |
N/A |
| Sensitivity (LOD) |
0.7 µg/mL (Native fluorescence)
3.0 µg/mL (Absorbance) (Values based on a monoclonal antibody) |
0.3 µg/mL
(Value based on internal standard) |
0.6 μg/mL (value based on internal standard) |
N/A |
| Sample Tray Options |
96-well plates or 48 vials |
96-well plates or 48 vials |
96-well plates or 48 vials |
96-well plates only |
| Power |
100 V-240 V (AC), 50/60 Hz, 500 W |
100 V-240 V (AC), 50/60 Hz, 500 W |
100 V-240 V (AC), 50/60 Hz, 500 W |
100 V-240 V (AC), 50/60 Hz, 500 W |
| Voltage Range |
0–6,500 V |
0–6,500 V |
0–6,500 V |
0–6,500 V |
| Temperature Control Range |
4-25 °C |
4-25 °C |
4-25 °C |
10-25 °C |
| Dimensions |
44 cm H x 42 cm W x 61 cm D |
44 cm H x 42 cm W x 61 cm D |
44 cm H x 42 cm W x 61cm D |
44 cm H x 42 cm W x 61 cm D |
| Weight |
46 kg (100 lb) |
46 kg (100 lb) |
46 kg (100 lb) |
46 kg (100 lb) |